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Spectrometers

The laboratory has two NMR spectrometers operating at a proton resonance frequency of 400 MHz and 600 MHz, respectively. The 600 MHz spectrometer is equipped with QCI CryoProbe (enabling simultaneous decoupling on multiple nuclei, such as, 13C, 31P and 15N) with cold preamplifiers that increase the sensitivity by a factor of four, being specially devoted to the determination of structures of biomolecules or small molecules at low concentrations and metabolomic studies. The 400 MHz spectrometer is equipped with a a TXI probe-head with inverse detection, a BBFO broadband and a HR MAS probe-heads, this latter enabling the acquisition of high-resolution NMR spectra of semi-solid (e.g.: paste, gels, resins, etc.) or liquid samples, from biological, organic or inorganic sources.

Bruker Avance III 400 MHz

rmn002

Description of the equipment:
- Console Avance III
- Three radiofrequency channels
- SampleXpress with 60 positions carousel for automatic sample changing
- BCU Xtreme for temperature range between -50 ºC and 180 ºC
- 5 mm BBFO plus broadband probe-head for direct observation of X with automated tuning and matching device (ATM).
- 5 mm TXI probe-head with inverse detection (optimized for 1H observing), designed to operate at three fixed frequencies (1H, 13C and 15N), with pulse-field gradients along Z axis and automatically tunable.
- HR MAS Triple H/C/N/D (4mm) high-resolution triple resonance probe-head with Magic Angle Spinning rotation.
- Topspin V.3.0 software
- DOSY module for processing DOSY experiments

Description of the equipment:
– Console Avance III
– Three radiofrequency channels
– SampleXpress with 60 positions carousel for automatic sample changing
– BCU Xtreme for temperature range between -50 ºC and 180 ºC
– 5 mm BBFO plus broadband probe-head for direct observation of X with automated tuning and matching device (ATM).
– 5 mm TXI probe-head with inverse detection (optimized for 1H observing), designed to operate at three fixed frequencies (1H, 13C and 15N), with pulse-field gradients along Z axis and automatically tunable.
– HR MAS Triple H/C/N/D (4mm) high-resolution triple resonance probe-head with Magic Angle Spinning rotation.
– Topspin V.3.0 software
– DOSY module for processing DOSY experiments

Bruker Avance III 600 MHz

rmn001Description of the equipment:
– Console Avance III
– Four radiofrequency channels
– SampleJet (carousel and robotic arm for automatic sample changing) with 96 positions for NMR tubes and five positions for well-plate sized NMR tube racks that allow 500 samples tubes in cold state for metabolic studies.
– BCU Xtreme for temperature range between -60 ºC and 180 ºC
– QCI CryoProbe with four independent channels that enable simultaneous decoupling on multiple nuclei, such as 13C, 31P and 15N. It is equipped with cold preamplifiers for 1H and 13C and the cryogenically cooled preamplifier for deuterium.
– Topspin V.3.0 software
– Metabolomic software for processing metabolic experiments

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